Catalog Number - 59018-25P

MicroBee C18™ is a traditional octadecylsilane (C18) reversed-phase HPLC stationary phase designed for laboratories seeking reliable performance and excellent value. As part of the Cogent™ Similars product line, it has been widely used for many years as a cost-effective alternative to legacy C18 columns specified in established analytical methods. The end-capped, Type-A silica support is bonded with a straight-chain C18 ligand, providing the hydrophobic retention characteristics expected from classic reversed-phase chromatography.

59018-25P is a 250 mm x 4.6 mm ID analytical column packed with 10 µm, 125Å MicroBee C18 particles. The 250 mm length provides greater resolving power and increased theoretical plates than shorter columns, making it well suited for complex separations, impurity profiling, method development, and applications where maximum resolution is more important than analysis speed. The industry-standard 4.6 mm ID format typically operates optimally at approximately 1.0–2.0 mL/min on conventional HPLC systems.

This stationary phase is an excellent alternative for methods specifying USP L1 columns, including applications historically developed on Microbondapak™ and similar C18 columns. The hydrophobic C18 surface is well suited for neutral and non-polar compounds and is particularly effective for the analysis of long-chain fatty acids, lipophilic compounds, pharmaceutical ingredients, and other compounds retained through reversed-phase mechanisms.

Key Benefits

• Traditional end-capped C18 stationary phase

• Suitable alternative for USP L1 methods

• Increased resolution from 250 mm column length

• Compatible with legacy C18 methods

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

• Excellent for hydrophobic and neutral compounds

• Effective for long-chain fatty acid analysis

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Method development

• Impurity profiling

• Long-chain fatty acid analysis

• Lipophilic compound separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee C18

• USP Classification: L1

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: C18 (Octadecylsilane)

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Traditional end-capped C18 chemistry

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Longer column length provides greater resolving power

• Excellent alternative for legacy USP L1 methods

• Suitable for hydrophobic and neutral compounds

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